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A two-site enzyme-linked immunosorbent assay (ELISA) for human smooth muscle myosin (smM) was developed and characterized. The purified human smM (FMC) was used as the primary antibody. SmM-specific polyclonal antibodies were prepared in rabbits with purified smM as the antigen. The assay is specific for human smM, with minimal cross-reactivity with other muscle proteins. It has low sensitivity for human serum, with a working concentration of approximately 0.2 ng ml-1 of human serum (10% serum). The assay could be reproduced with anti-smM polyclonal antibodies in various sources, with the exception of a single batch of the preparation from a single source. It could be reproduced for several months without loss of antibody activity. SmM-specific antibodies were also detected by indirect immunofluorescent microscopy, which confirmed their specificity in the ELISA.This $32 Raspberry Pi Touchscreen Isn’t Just for Kids
One of our favorite inventions to date is the Raspberry Pi. This tiny computer costs just $25 and allows you to create your own custom PC from the ground up. What’s even better is that you can snag one for just about every situation you can think of – like as a digital photo frame, a portable music player, or even a touchscreen monitor. What’s left to do? Put that touch screen on something.
Thanks to Vittoria Products, you can do just that. Their new Raspberry Pi Touch Screen v2 is a four-ounce screen that connects with the Raspberry Pi via a USB cable. No soldering needed.
So, if you’ve got any old touchscreens lying around, you can convert them into touchscreens for your Raspberry Pi. If not, the touchscreen can
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